Latest news (most recent on top)

New Bio-Kine32 version (Online May 18, 2012)

V4.66 available for download to registered users.

New instrument

SFM-series - The new world's best multimixer stopped flow - see here
(May 7, 2012)

New Bio-Kine32 version (Online March 19, 2012)

V4.65 available for download to registered users.

New accessory for the MOS-450/AF-CD

ORD (Optical Rotatory Dispersion) - see AN11 - ORD accessory

3D trace using a diode array in absorbance mode

New technical note - TN66
(for registered users - see here)

Bio-Kine32 Windows 7 compatibility

Bio-Kine32 is now compatible with Windows 7 (32 and 64 bits) - Access to download

A new approach to get structural and kinetics information simultaneously: SFM for Transient Time-resolved FRET measurements

Structural kinetics of myosin by transient time-resolved FRET
Yuri E. Nesmelov, Roman V. Agafonov, Igor V. Negrashov, Sarah E. Blakely, Margaret A. Titus, and David D. Thomas PNAS,
February 1, 2011, vol. 108, no. 5 (1891-1896)
(SFM-20/S + pulse laser coupling)

New instrument

SFM-100 / MOS-LED - All in one stopped-flow system - see here
(December 3, 2010)

A new example of Bio-Logic stopped-flow capabilities: detailed structural and electronic information on reaction intermediates are revealed by combining simultaneously ED-XAFS and time-resolved UV-vis spectroscopy

Multitechnique Approach to Reveal the Mechanism of Copper(II)-Catalyzed Arylation Reactions
Moniek Tromp, Gino P. F. van Strijdonck, Sander S. van Berkel, Adri van den Hoogenband, Martinus C. Feiters, Bas de Bruin, Steven G. Fiddy, Ad M. J. van der Eerden, Jeroen A. van Bokhoven, Piet W. N. M. van Leeuwen, and Diederik C. Koningsberger
Organometallics 2010, 29, 3085–3097
(SFM-400 + ED-XAFS+simultaneous diode array)

Single wavelength kinetics experiment using MOS-200 in absorbance mode

New technical note - TN64
(for registered users - see here)

Anisotropy T-format using MOS-200M and an additional PMS-250

New technical note - TN61
(for registered users - see here)

Pre-configured systems - Combining the highest performance and best prices.

For many years Bio-Logic has led the industry in providing the highest quality and best performing rapid kinetics systems. We are now taking the lead in providing the same quality and performance, at prices that are incredibly competitive for today's user.

We have assembled packages containing our most popular combinations of stopped flow mixers and optical systems, and set package prices that are significantly lower than the total component prices.

The stopped flow and optical systems are identical to those available separately and are completely compatible with all of the same options and accessories.

The package systems allow you to greatly increase your range of applications for a system and stay within your budget.

For example, you could order an SFM-300/S and MOS-200 separately. Or, for a slightly lower total price, you can now get the SFM-300/S + MOS-200 package, and add the freeze quench accessory, and also add the quench flow upgrade for the SFM-300.

If you are planning to get a system in the near future, contact us now for a current quote including one of these packages and the options or accessories important for your applications.

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An review of state of the art stopped-flow measurements on synchrotron and neutron beam lines

Applications of stopped-flow in SAXS and SANS
Isabelle Grillo
Current Opinion in Colloid and Interface Science 14 (2009) 402-408
(SFM-300)

X-ray Head and Capillary holder

New technical note - TN60
(for registered users - see here)

Single wavelength kinetics experiment using MOS-200M/CD in CD mode

New technical note - TN59
(for registered users - see here)

New section in "Selection of scientific articles using the Bio-Logic rapid kinetics systems"

Stopped-flow (mT jump)

Frequently asked questions

Please take a look at our new FAQ section.

Our instruments in anaerobic conditions (updated March 1st, 2007)

Our instruments have been used in several occasions in anaerobic conditions.
For more information please click here

A Millisecond Infrared Stopped-Flow Apparatus
Jia Tang and Feng Gai Applied Spectroscopy, Vol. 60, Issue 12, pp. 1477-1481
(SFM-300 + FT-IR accessory)

More articles here

Titration of Cytochrome c using the titrator accessory

New technical note - TN58
(for registered users - see here)

Rapid Mixing Temperature Jump System

• Hot and cold jumps
• T-changes as high as several ten of degrees
• Low volume consumption
• Millisecond resolution
For more information please click here
See also our references using Stopped-flow (mT jump)

Participation to the EMBO Practical Course

Bio-Logic was pleased to participate to
"Transient Kinetics Methods Applied to Biological Macromolecules"
18th to 24th June 2006 at the Department of Biosciences, University of Kent, UK

Designing double mixing quench-flow experiments

New technical note - TN52
(for registered users - see here)

Stopped-flow conductimetry

A new detection mode for Rapid-Kinetics
For more information please click here (PDF file)

Rapid-Kinetics experiments down to -90C°

The cryo-stopped-flow assembly extends the range of operation of the Bio-Logic stopped-flow instruments to far sub-zero temperatures. It allows rapid kinetics experiments to be performed down to -90°C." - Click here (PDF file) for more information

Faster sampling rate for Bio-Kine32

Due to the constant increase in speed of the Bio-Logic Stopped-Flow instruments, the data acquisition rate of Bio-Kine32 in transient recording mode has been increased to 100 kHz (10 µs per data point). - Click here for more information

Special mixing of viscous solutions

In our all our Stopped-Flow instruments, syringe velocity is programmed and thus is independent of the solution viscosity. Very asymmetric mixing such as pure glycerol against water can therefore be achieved... - Click here for more information

New specification for our SFM-300 & SFM-400

0.25 ms dead time with the new microvolume accessory
Click here for more information

From Neutron Scattering & Synchrotron Radiation to Microgravity experiments in parabolic flights

Bio-Logic instruments are today the N°1 choice for experiments in the most stringent conditions.

• Click here for information on Synchrotron Radiation / Neutron Scattering experiments
• Click here for information on Microgravity experiment

Improved and simplified control of stepping motor with new software driver

A new stopped-flow software driver is now available (MPS32 V2.02). In addition to the classical interface it now offers an advanced mode that provides improved and simplified control of stepping motor action resulting in high precision mixing ratio and reproducibility. In addition, this new mode of control offers full users assistance in the instrument usage. It is highly recommended that SFM-300/400 and SFM-20 users download this last version of the stopped-flow driver - click here for more details.

Titrator and Peltier element controller

Because of its extreme precision of volume delivery, the Bio-Logic stopped-flow instruments feature the most accurate titrator of the market. By a simple software choice and an easy hardware installation, any SFM-300/400 or SFM-20 can be converted in a high precision two syringe titrator. The titrator device also comes in a version that includes a Peltier Cell temperature cycler. This Peltier/titrator system benefit from a full integration with Spectrometer control software (Bio-Kine32). As such it can easily be used in absorbance, fluorescence, fluorescence anisotropy and circular dichoism.

New plungers and valves

The old styled Teflon plunger and the old valves have been replaced by a new type of plunger with o-ring and a new type of valve with o-rings. The result obtained is a perfect seal whatever the temperature excursion is.

New hardstop valve

Small theoretical deadtime is one thing ! However the quality of the real "stop" is more important: in many cases "stop" artifacts prevent users from analyzing the first data points of the Kinetics. We are now using a specially designed "hardstop" valve for a quasi perfect stop.

New articles

• Structural kinetics of myosin by transient time-resolved FRET
  
• Multitechnique Approach to Reveal the Mechanism of Copper(II)-Catalyzed Arylation Reactions
• Rapid Collapse into a Molten Globule Is Followed by Simple Two-State Kinetics in the Folding of Lysozyme from Bacteriophage λ
• Evaluation of proton activity in microemulsions by a kinetic probe
• Characterization of Dehaloperoxidase Compound ES and Its Reactivity with Trihalophenols
  
• Biophysical characterization reveals structural disorder in the developmental transcriptional regulator LBH
• Growth Mechanisms in Nanocrystalline Lead Sulfide by Stopped-Flow Kinetic Analysis
• Characterization of Dehaloperoxidase Compound ES and Its Reactivity with Trihalophenols
• Fluorometric assays for characterizing DNA helicases
• Hg2+-Reactive Double Hydrophilic Block Copolymer Assemblies as Novel Multifunctional Fluorescent Probes with Improved Performance
• Kinetics of Complex Formation between DNA and Cationically Charged Cylindrical Brush Polymers Observed by Stopped Flow Light Scattering
• pH (low) insertion peptide (pHLIP) inserts across a lipid bilayer as a helix and exits by a different path
• Temperature and Solvent Effects on Radical Scavenging Ability of Phenols
• Automated Cell-Based Assay for Screening of Aquaporin Inhibitors
• Comparative Study of Temperature-Induced Association of Cyclic and Linear Poly(N-isopropylacrylamide) Chains in Dilute Solutions by Laser Light Scattering and Stopped-Flow Temperature Jump
• A Calibration Reaction for Rapid Freeze-Quench W-Band EPR
• Applications of stopped-flow in SAXS and SANS
• Binding kinetics of glucose and allosteric activators to human glucokinase reveal multiple conformational states
• Local structure of reaction intermediates probed by time-x-ray absorption near edge structure spectroscopy
• The mechanism of addition of pyridoxal 5´-phosphate to Escherichia coli apo-serine hydroxymethyltransferase
• An allylic ketyl radical intermediate in clostridial amino-acid fermentation
• Distinct Unfolding and Refolding Pathways of Ribonuclease A Revealed by Heating and Cooling Temperature Jumps
• Micellization Kinetics of a Novel Multi-Responsive Double Hydrophilic Diblock Copolymer Studied by Stopped-Flow pH and Temperature Jump
• Cytochrome b5 Increases the Rate of Product Formation by Cytochrome P450 2B4 and Competes with Cytochrome P450 Reductase for a Binding Site on Cytochrome P450 2B4
• Key feature of the catalytic cycle of TNF-α converting enzyme involves communication between distal protein sites and the enzyme catalytic core
• A semiquinone intermediate generated at the Qo site of the cytochrome bc1 complex: Importance for the Q-cycle and superoxide production
• Identification of Alpha-1 Acid Glycoprotein as a Lysophospholipid Binding Protein: A Complementary Role to Albumin in the Scavenging of Lysophosphatidylcholine
• Biochemical Characterization and Ligand Binding Properties of Neuroglobin, a Novel Member of the Globin Family
• Characterization of human D-amino acid oxidase
• Reduction of ferric haemoproteins by tetrahydropterins: a kinetic study
• A Millisecond Infrared Stopped-Flow Apparatus
• Covalent heme attachment in Synechocystis hemoglobin is required to prevent ferrous heme dissociation
• Multistate reaction kinetics of 6-hydroxy-4-(dimethylamino)flavylium driven by pH. A stopped-flow study
• Specific Aquaporins Facilitate the Diffusion of Hydrogen Peroxide across Membranes
• Escherichia coli RecQ Is a Rapid, Efficient, and Monomeric Helicase
• The Formin Homology 1 Domain Modulates the Actin Nucleation and Bundling Activity of Arabidopsis FORMIN1
• RecQ Helicase-catalyzed DNA Unwinding Detected by Fluorescence Resonance Energy Transfer
• Revisiting a proposed kinetic model for the reaction of cysteine and hydrogen peroxide via cysteine sulfenic acid
• Structural Characterization of Apomyoglobin Self-Associated Species in Aqueous Buffer and Urea Solution
• Structural Insights into the Functional Interaction of KChIP1 with Shal-Type K+ Channels
• In situ investigation of the oxidative addition in homogeneous Pd catalysts by synchronised time resolved UV-Vis/EXAFS
• Amyloid formation under physiological conditions proceeds via a native-like folding intermediate
• Polymerization of Anionic Wormlike Micelles
• pH-Induced Micellization Kinetics of ABC Triblock Copolymers Measured by Stopped-Flow Light Scattering
• A Stopped-Flow Kinetic Study of the Assembly of Interpolymer Complexes via Hydrogen-Bonding Interactions
• First Observation of Two-Stage Collapsing Kinetics of a Single Synthetic Polymer Chain
• Mechanistic Information from Low-Temperature Rapid-Scan and NMR Measurements on the Protonation and Subsequent Reductive Elimination Reaction of a (Diimine)platinum(II) Dimethyl Complex
• Mechanism of a-oxoamine synthases: identification of the intermediate Claisen product in the 8-amino-7-oxononanoate synthase reaction
• Probing the Kinetics of Membrane-Mediated Helix Folding
• Time-resolved scattering investigations of brome mosaic virus microcrystals appearance
• Efficient Refolding of Aggregation-prone Citrate Synthase by Polyol Osmolytes HOW WELL ARE PROTEIN FOLDING AND STABILITY ASPECTS COUPLED?*
• Ribose 2'-hydroxyl groups in the 5' strand of the acceptor arm of P-site tRNA are not essential for EF-G catalyzed translocation
• The mechanism of GroEL/GroES folding/refolding of protein substrates revisited
• Trifluoroethanol-Induced Unfolding of Concanavalin A: Equilibrium and Time-Resolved Optical Spectroscopic Studies
• Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis
• Structural and kinetics characterization of the simplified SH3 domain FP1
• Stabilization of Native Protein fold by intein-mediated covalent cyclization
• Computational redesign of protein-protein interaction specificity
• Escherichia coli RecQ Is a Rapid, Efficient, and Monomeric Helicase
• Evidence of Two Distinct Oxygen Complexes of Reduced Endothelial Nitric Oxide Synthase
• Pore selectivity analysis of an aquaglyceroporin by stopped-flow spectrophotometry on bacterial cell suspensions.
• Probing the Reorganization of the Nicotinic Acetylcholine Receptor during Desensitization by Time-Resolved Covalent Labeling Using [3H]AC5, a Photoactivatable Agonist.
• RecQ Helicase-catalyzed DNA unwinding detected by fluorescence resonance energy transfer
• The Heparin Binding Properties of Heparin Cofactor II Suggest an Antithrombin-like Activation Mechanism
• Rapid mixing methods for exploring the kinetics of protein folding
• Cooperative Sub-Millisecond Folding Kinetics of Apomyoglobin pH 4 Intermediate
• Dynamics of structural transitions in amphiphilic systems monitored by scattering techniques
• An advanced EPR stopped-flow apparatus based on a dielectric ring resonator
• Stopped-flow and Mutational Analysis of Base Flipping by the Escherichia coli Dam DNA-(adenine- N6)-methyltransferase
• Steps towards Phage Display Libraries with an extended amino acid repertoire
• DNA recognition by the Homing Endonuclease PI-SceI involves a divalent metal ion cofactor induced conformational change
• Apo-azurin folds via an intermediate resembles the moten-globule
• Probing the influence on folding behavior of structurally conserved core residues in P.Aerogonesa apo-azurin
• Acid-base behavior of organopalladium complexes [Pd(CNN)R]BF4
• Two coupled photochromic systems of 3',4'-(methylendioxy)flavilium : kinetic and thermodynamic characterisation
• Properties of some variants of human beta2-microglobulin and amyloidogenesis
• Human Rhesus-associated glycoprotein mediates facilitated transport of NH3 into red blood cells
• Roles of conserved P domain residues and Mg2+ in ATP binding in the ground and Ca2+ activated states of sarcoplasmic reticulum Ca2+ - ATPase
• Glutamate-183 in the conserved TGES motif of domain A of sarcoplasmic reticulum Ca2+ - ATPase assists in catalysis of E2/E2P partial reactions
• Importance of transmembrane segment M1 of the sarcoplasmic reticulum Ca2+-ATPase in Ca2+ occlusion and phosphoenzyme processing
• Roles of Leu249, Lys252 and Leu253 in membrane segment M3 of sarcoplasmic reticulum Ca2+-ATPase in control of Ca2+ migration and Long-range intramolecular communication
• Dissection of the functionnal differences between sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) 1 and 2 isoforms and characterization of darier disease (SERCA2)mutants by steady-state and transient kinetic analysis
• Importance of conserved N-domain residues thr441, Glu442, lys515, Arg560, Leu562 of sarcoplasmic reticulum Ca2+-ATPase for MgATP binding and subsequent catalytic steps
• Importance of conserved Thr214 in domain A of the Na+, K+ - ATPase for stabilization of the phosphoryl state complex in E2P dephosphorylation
• Functional consequences of alterations of Ile279, Ile283, Glu284, his285, Phe286, and His288 in the NH2-terminal part of the transmembrane helix M3 of the Na+, K+ - ATPase M.
• Dissection of the functionnal differences between sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) 1 and 3 isoforms by steady-state and transient kinetic analysis
• Importance of Glu282 in transmembrane segment M3 of the of the Na+,K+-ATPase for control of the cation interaction and conformational changes
• Importance of transmembrane segment M3 of the sarcoplasmic reticulum Ca2+-ATPase for control of the gateway to the Ca2+ sites
• Importance of Thr-353 of the conserved phosphorylation loop of the sarcoplasmic reticulum Ca2+-ATPase in MgATP binding and catalytic activity
• Mutational effects on conformational changes of the dephospho-and phospho-forms of the Na+,K+-ATPase
• Importance of stalk segment S5 for Intramolecular communication in the sarcoplasmic Ca2+-ATPase 
• Revisiting the role of H+ in chmotactic signaling of sperm
• Facile Variation of Reagent Concentrations in Rapid Quench Enzymology.
• The signal flow and motor response controling chemotaxis of sea urchin sperm.
• Structural reorganization of the Acetylcholine binding site of the Torpedo nicotinic receptor as revealed by dynamic photoaffinity labeling
• Enzymatic mechanism of RNA translocation in dsRNA bacteriophages
• Amino Acids of the Bacterial Toxin SopE Involved in G Nucleotide Exchange on Cdc42
• The influence of hinge region residue Glu381 on antithrombin allostery and metastability.
• Catalytic Mechanism of the Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26.
• Kinetic Analysis of R67 Dihydrofolate Reductase Folding: From the Unfolded Monomer to the Native Tetramer
• Kinetic Characterization of the pH-Dependent Oligomerization of R67 Dihydrofolate Reductase
• Role of individual disulfide bonds in hen lysozyme early folding steps
• Immunochemical pulsed-labeling characterization of intermediates during hen lysozyme oxidative folding
• How does ZrO2/surfactant mesophase nucleate? Formation mechanism
• Formation and growth of anionic vesicles followed by small angle neutron scattering
• The complex between a four-way DNA junction and T7 endonuclease 1
• Mapping the Cytochrome c Folding Landscape
• Surface Expansion Is Independent of and Occurs Faster than Core Solvation during the Unfolding of Barstar
• DNA: REPLICATION REPAIR AND RECOMBINATION: Kinetic Mechanism for the Formation of the Presynaptic Complex of the Bacterial Recombinase RecA
• Characterization of Single-Tryptophan Mutants of Histidine-Containing Phosphocarrier Protein: Evidence for Local Rearrangements during Folding from High Concentrations of Denaturant
• Phosphorylation of the WASP-VCA Domain Increases Its Affinity for the Arp2/3 Complex and Enhances Actin Polymerization by WASP 
• Metal ions bound at the active site of the junction-resolving enzyme T7 endonuclease I.
• Understanding protein hydrogen bond formation with kinetic H/D amide isotope effects

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